Embryo-Safe Disinfectant Spray

Fertisafe™ has become the disinfectant of choice for ART labs and theatres that want to disinfect work areas and lab equipment between procedures, without exposing samples to harmful Volatile Organic Compounds (VOCs).

Fertisafe™ contains a unique blend of chlorine, hydrogen and oxygen compounds (including chlorine dioxide, hypochlorites, ozone and free oxygen radical).  Unlike a simple chlorine solution, it has a low chemical load and does not react with the nitrogen products in biomasses, but is very potent at the molecular level against microbial structures.


“I have been routinely using Fertisafe™ products in the IVF lab and find them to be VOC free, non-toxic to mouse gametes and pre-implantation embryos and extremely effective.”
Graham Coull, Sims IVF, Dublin, Ireland


Key Features

Embryo Safe

MEA tested, alcohol and VOC free, Quaternary ammonium (DDAC) free

Laboratory Safe

Safe for use on laboratory grade stainless steel.  No toxic residues after disinfecting


Bactericidal and virucidal in seconds.  Fungicidal and sporicidal in minutes


Biodegradable, eco-friendly solution for disinfection


Each batch of Fertisafe™ is tested for efficacy against Bacillus subtilis var niger and achieves a 6-log reduction in five minutes


Order Code Product
7-90-305 Fertisafe™ Spray (1 litre bottle)

Please download the Fertisafe Efficacy Table here


Fertisafe™ Publications

Key Publications IconWhite Paper (2007) Using Fertisafe™ in Incubators

Key Publications IconJanssens, R. et al. (2007) “Novel sterilizing solution Fertisafe™ is effective and non embryotoxic”. Human Reproduction. Vol 22 / Aug 2007. p.221

Key Publications IconMaher, B. (2008) “Lab disinfectant harms mouse fertility”. Nature. Vol 453/19 June 2008. p.964

Order Codes

Order Code Product
7-90-305 Fertisafe™ Spray (1 litre bottle)


Q: Is Fertisafe™ safe for embryos?
A: The safety of each batch of Fertisafe™ is routinely confirmed through MEA testing.

Q: What research has been done on Fertisafe™?
A: Please refer to the abstract of R. Janssens’ paper published at ESHRE 2007 and linked on the RI website. In the study, the spray was used in a controlled environment and colony-forming units were counted after a 48-hour period. The researchers concluded that: “Fertisafe™ can be safely used in IVF laboratories, as it was proved to be an effective decontamination solution while being non-toxic for gametes and pre-implantation embryos?”

Abstract available on the webpage:

Janssens R, Cortvrindt R, Devroey P, Lauwers S, Van der Elst J, 2007. Novel sterilizing solution Fertisafe™ is effective and non embryo toxic. In: ESHRE (European Society for Human Reproduction and Embryology)23rd annual meeting. Lyon, France 1-4 July 2007

Q: How is Fertisafe™ proven embryo-safe?
A: The spray is MEA tested by humidifying the surface of a Petri dish with the Fertisafe™ liquid and the liquid is allowed to completely evaporate before use.

1.1. Zygotes are incubated in a treated collection dish for 30 minutes before transfer to the culture dish.

1.2. Culture plate is treated as described above and used for grouped embryo culture thereafter.

The resulting embryos develop in line with the control group not exposed to Fertisafe™. MEA test certificates are available.

Q: How long after application is the surface safe for resuming activities?
A: The surface is ready for use after Fertisafe™ (or the water wash) is dry.

Q:What organisms are susceptible to Fertisafe™?
A: Fertisafe™ is composed of chlorine, sodium, hydrogen and oxygen compounds which rapidly destroy bacteria, viruses, spores and fungi.

Q: What is the active ingredient?
A: Fertisafe™ is electrolyzed brine. It is produced by passing dissolved sodium chloride (brine) across electrodes in combination with a patented membrane technology. This produces a range of chlorine, sodium, hydrogen and oxygen compounds that, unlike a pure chlorine solution, has a low chemical load and does not react with the nitrogen products in biomasses. It is very potent at the molecular level against microbial structures.

Q: I smell chlorine – is this right?
A: Fertisafe™ contains a tiny amount of free chlorine – enough to smell, but it’s not harmful.

Q: Does Fertisafe™ eliminate HIV, Hepatitis B (HBV) and Hepatitis C (HCV)?
A: Fertisafe™ has been tested on HIV Virus Type 1; strain HTLV-IIIB in accordance with the US Environmental Protection Agency (EPA) DIS / TSS-07 guidelines. Complete inactivation of HIV –1 was achieved after a 10 minute exposure time to Fertisafe™. This fully meets the EPA’s requirements. It also eliminates both the Hepatitis B and C viruses.

Q: Is Fertisafe™ also safe to use near sperm?
A: As with all disinfectants, direct contact, or use in a very confined space with Fertisafe™ is toxic to sperm. However, unlike other disinfectants, Fertisafe™ has no harmful effects once dried.

Q: Can I use Fertisafe™ to disinfect my incubator?
A: RI is currently working on this and has commissioned a white paper on the subject. The paper is available upon request.

Q: Can Fertisafe™ be used on floors?
A: Some laboratories do use it for this purpose, undiluted or diluted 5:1.

Q: A white patchy residue has been left behind. What is it, is it safe and how do I remove it?
A: This is simply salt (sodium chloride) and is harmless. If you wish to remove it, use sterile water and a sterile dry wipe to clean the residue away.

Q: Can I immerse items in Fertisafe™ liquid?
A: We recommend Fertisafe™ be used as a spray.

Q: How do I store Fertisafe™?
A: Fertisafe™ should be stored away from direct sunlight and away from heat.

Q: What is the recommended frequency of use?
A: It should be used daily to disinfect workbenches, microscopes and all work areas in the laboratory. Disinfecting is up to individual lab protocols but many clinics opt to use it between each procedure.

Q: How long does a one litre bottle of spray last?
A: This depends on how big the lab is and the frequency it is used. An average size lab would use approximately one bottle per month.

Q: Once opened, how long can the product be used for?
A: 4 weeks.

Q: How effective is Fertisafe™?
A: Fertisafe™ is batch tested with a biological surrogate (biologic indicator) – Bacillus subtilis var. niger spores. Fertisafe™ achieves a 6-log reduction. This means that Fertisafe™ will reduce the number of spores to one millionth of the original level. This is as close to the theoretical ideal of zero microbes remaining as is practically achievable, and is the gold standard pass mark for all sterilisation and disinfection processes.

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