Integra 3 Micromanipulator

Integra 3TM

The world’s most advanced micromanipulation system

Every detail of the Integra 3™ is geared towards one goal – to ensure you create the best possible conditions for ICSI and sample manipulation.

We recognise that temperature and time are critical within an ART lab.  Integra 3™ is ultra-responsive and effortlessly smooth, so it’s quick and easy to use, thanks to RI’s advanced mechanical design.  You can change micropipettes in seconds, which can transform the efficiency of your lab overnight.

We have increased the size of the integrated heated stage by 40% but kept the footprint to a minimum to keep your work area uncluttered.

“By far the best new feature is the warm air flow through the central plate – which means your dish can maintain the correct temperature throughout the procedure. The egg counter feature has also been a blessing in large cases, as well as the warning “beeps” for the fine manipulator height indicator”
Sophie Bird
Embryologist, Guy’s and St.Thomas’ NHS Foundation Trust

Thermosafe™ is an integral heating system that helps to keep samples at the optimum temperature. It emits warm air precisely and uniformly towards the Petri dish.

The result of this is a fail-safe guarantee, with no more cold spots, no more hot spots and no more ambiguity.

There is also a stage temperature health indicator light to reassure you that the integrated heating system is functioning correctly.

ThermosafeTM – Ultimate Thermal Control for Integra 3TM

WithThermosafe

Thermal image of Petri dish surface when placed on heated metal plate WITH THERMOSAFE

withoutThermosafe1

Petri dish surface when placed on heated metal plate WITHOUT THERMOSAFE™

withoutThermosafe

Petri dish surface when placedon market leading glass ITO WITHOUT THERMOSAFE™

thermosafeTemperature

integra_full_setup

Key Features

No Cold Spots

Thermosafe™ maintains sample temperature with a gentle stream of warm air under the Petri dish

Super-Fast

Improved rapid micropipette set-up with one-step angle adjustment

Practical

Motion sensor LED light underneath the stage for improved visibility when changing objectives

Safe

Heating system status indicator light and alarm monitors every second of the process.  A log is kept of every alarm triggered

Integrated

Large built-in mechanical XY stage plus temperature controllers with easy access buttons and touch screen

Accurate

New extra-fine mechanisms for smooth and precise movement of the stage and micropipettes

Integra 3TM
The Definitive Micromanipulator

We’ve added a whole spectrum of other functions and features that make the Integra 3™ the most intuitive and natural mechanical micromanipulation system the world has ever known. View our Integra 3™ film to find out more.

More Giant Steps in the World of Micromanipulation

Motion Triggered LED Light

A motion triggered light illuminates under the stage as soon as it detects your hand, to help you see when changing objectives.

Colour Touch Screen

Using the 3.5 inch touch screen display, you can check temperature, track time with a stop watch and count the number of injections done.  The electronic height indicators track the vertical position of the toolholders.

Shortcut Video And Images

If you need to keep track of samples or procedures, just hit the shortcut buttons to film or photograph any stage of the process.  There’s a counter button and an onscreen stopwatch as well.

Precise And Intuitive Control

When you perform ART micromanipulation procedures, accuracy is paramount.  Integra 3™ offers the finest possible movement on the XYM stage and fine and coarse levers as well as a choice of oil or air syringes for the control of samples.

RI ViewerTM Software

You can use the Integra 3™ without a computer or camera.  However, if you connect it to a computer, we can supply the RI Viewer™ software. With RI Viewer™ you can use the shortcut keys.  Enjoy razor-sharp full screen imaging from the microscope, with digital magnification and a modern, clean user interface. It also offers line measurement tools and a built-in simulator for training and demonstration purposes.  RI Viewer™ also allows you to sync up with Saturn™ laser and RI IMSI™ systems.

Syringes

The Integra 3™ includes two of our SAS air syringes as standard.  These syringes offer superb control and eliminate the need for oil. Also available is the SAS-SE for superfine air control or our SOS oil syringe with a quick fill feature for minimum set-up time.

Microscope Compatibility

NIKON

TMD, Diaphot 200/300 TE2000, TE200/300, Ti

LEICA

DMIRB, DMI3000B/4000B/6000B DM IL, DMi8

OLYMPUS

IMT2, IX50/70 IX51/71/81, IX53/73/83

ZEISS

Axiovert 40/100/200 Axio Observer, Axio Vert.A1

* Contact us if your microscope is not listed

Specifications

Heating System
Thermosafe™ Air Heating System with Heated Metal Insert:
25mm aperture (standard)
16mm aperture (optional)
ITO Heated Glass Insert (optional)
Temperature Controller
Accuracy: better than ±0.1ºC when calibrated against known reference
Resolution: 0.1ºC
Setpoint Temperature Range: 30-45ºC
Display
3.5″ colour LCD touch screen display for temperature control
Connectivity
USB socket for connection to PC running RI Viewer™ software
Syringes
Choice of SAS (air), SAS-SE (air) and SOS (oil)
Weight
Maximum weight of microscope mounted components (excluding front and rear adaptors): 10.4kg
Actual weight is configuration dependent
Dimensions
Footprint (not including microscope): width 56cm, depth 38cm
Power Supply
Input: 100-240VAC, 50-60HZ, Max. 1.8A, Class 1
Output: 12VDC, Min. 11A (132W)
Operating Range
Temperature: 15ºC (59ºF) to 40ºC (104ºF)
Humidity:15% to 85% RH (non condensing)
Micropipette Compatibility
Compatible with Research Instruments micropipettes (or other industry standard 1.0mm outside diameter micropipettes or equivalent specification).

Order Codes

Please use the table below to select the correct part number for your equipment.

For Saturn Fixed , other microscopes and any other system not covered by the part numbers below, contact RI Orders by emailing orders@research-instruments.com

Microscope Manufacturer Model Syringe Choice Part Number Description
Nikon Ti-S, Ti-U, Ti-E
TE2000,TE200, TE300
None 6-54-100-01 Integra 3 Thermosafe Nikon
No Syringes
Nikon Ti-S, Ti-U, Ti-E,TE2000,TE200, TE300 2 x SAS 6-54-100-02 Integra 3 Thermosafe Nikon
2 x SAS
Nikon Ti-S, Ti-U, Ti-E,TE2000,TE200, TE300 1 x SAS,
1 x SAS-SE
6-54-100-03 Integra 3 Thermosafe Nikon
1 x SAS , 1 x SAS-SE
Nikon Ti-S, Ti-U, Ti-E,TE2000,TE200, TE300 2 x SAS-SE 6-54-100-04 Integra 3 Thermosafe Nikon
2 x SAS-SE
Olympus IX53, IX73, IX83,IX51, IX71, IX81 None 6-54-100-05 Integra 3 Thermosafe Olympus
No Syringes
Olympus IX53, IX73, IX83,IX51, IX71, IX81 2 x SAS 6-54-100-06 Integra 3 Thermosafe Olympus
2 x SAS
Olympus IX53, IX73, IX83,IX51, IX71, IX81 1 x SAS,
1 x SAS-SE
6-54-100-07 Integra 3 Thermosafe Olympus
1 x SAS, 1 x SAS-SE
Olympus IX53, IX73, IX83,IX51, IX71, IX81 2 x SAS-SE 6-54-100-08 Integra 3 Thermosafe Olympus
2 x SAS-SE
Olympus IX50, IX70 2 x SAS 6-54-100-13 Integra 3 Thermosafe Olympus IX1
2 x SAS
Leica DMi8, DMIx000B, DMIRB None 6-54-100-09 Integra 3 Thermosafe Leica
No Syringes
Leica DMi8, DMIx000B, DMIRB 2 x SAS 6-54-100-10 Integra 3 Thermosafe Leica
2 x SAS
Leica DMi8, DMIx000B, DMIRB 1 x SAS,
1 x SAS-SE
6-54-100-11 Integra 3 Thermosafe Leica
1 x SAS, 1 x SAS-SE
Leica DMi8, DMIx000B, DMIRB 2 x SAS-SE 6-54-100-12 Integra 3 Thermosafe Leica
2 x SAS-SE
Leica DMi8, DMIx000B, DMIRB 2 x SAS 6-54-110-01 Integra 3 No Thermosafe Leica
2 x SAS
Leica DMi8, DMIx000B, DMIRB 2 x SAS 6-54-110-12 Integra 3 ITO Leica
2 x SAS

FAQs

Q. What are the advantages of the Integra 3™?
A. The Integra 3™ has very natural and intuitive controls. It has a quick and easy micropipette set-up procedure, with the ability to change the micropipette angle with a single control. It is also compact and takes up very little laboratory bench-top space, whilst providing a large stage surface area to work on. Thermosafe™ ensures consistent temperature, including in the microscope line of sight, to provide stable temperature during micromanipulation.

Q. How easy is it for a customer to change over to RI if they have never used the Integra 3™ before?
A. Very easy – most people convert perfectly after a couple of sessions. You have to get used to new hand positions, but this takes very little time and once mastered, the control is superb and the operation much quicker.

Q .What is included in the system?
A. The Integra 3™ comes as standard with: 2 x micromanipulators incorporating coarse and fine controls, 2 x toolholders with easy micropipette set-up, 1 x large heated stage, 1 x Thermosafe™ warm air heating system (option available without Thermosafe™), 1 x 3-channel temperature controller with improved touch screen control, 2 x SAS syringes / injectors, 2 x handrests, 1 x mechanical XY stage.  Optional extras: Glass heated stage insert, extra-smooth air syringe (SAS-SE) or oil syringe (SOS), microscope camera, PC / monitor, RI imaging software RI Viewer™.

Q. What if we wish to use existing syringes or syringes from another company?
A. We have adaptors which allow existing syringes to be used with the Integra 3™.

Q. Can RI provide training?
A. We can provide instruction on how to use the equipment at installation, and can also arrange a training session at a current user’s lab, or arrange for an experienced embryologist to visit your lab. This is equipment training only. If clinical procedure training is needed, we can put you in touch with laboratories who offer this service.

Q. Why would Thermosafe™ be useful?
A. Maintaining even temperatures during micromanipulation helps preserve the best conditions for the samples even outside of the incubator. Please refer to “Can temperature stability be improved during micromanipulation procedures by introducing a novel air warming system?”, 2014, Wheat S, Vaughan K, Harbottle S.” and Integra TI vs Integra 3 (I3): a prospective randomised sibling study assessing fertilisation outcome, embryo quality and morphokinetic parameters”, 2014, Gwinnett D, Cook C, Escriba M, Hickman C, Trew G, Lavery S.

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