Saturn 5TM

Fixed and Directional Laser Systems

As the pioneer of moveable lasers for Assisted Reproductive Technology, the Saturn 5 Active™ laser is spearheading a revolution in hatching and biopsy techniques in laboratories all over the world.

The Saturn 5™ Biopsy Mode is helping you to significantly improve biopsying methods; potentially reducing procedure time, lowering the incidence of blastocyst collapse and eliminating the need to mechanically tear off cells.

Using Biopsy Mode, you can draw a straight or curved line along the sample, select the number and size of holes, then simply “fire”. The laser will ablate exactly along the chosen path, meaning you no longer need to move the holding pipette at all.  It is that easy.

“We have been using the Saturn 5 Active™ laser system for assisted hatching, blastomere biopsy and blastocyst collapsing since November 2012. The moveable Saturn™ laser means I can ablate the zona exactly where I want to, without having to move the embryo, also the new biopsy mode allows me to accurately make holes in either straight or curved lines along the zona. The new laser system from RI makes the procedures quicker, easier and safer to perform than ever before. We love it!”
Samantha Knight
SPIRE London Fertility Clinic, UK
Saturn 5TM
Fixed and Directional Laser Systems

As the Saturn 5™ pilot laser travels down the same fibre optic path as the ablation laser, it guarantees consistent positioning.

You can calibrate and verify hole size and firing position with sub-micron accuracy simply and quickly, leaving more time for your procedures.

Key Features

Faster Biopsies

Biopsy mode allows accurate laser drilling along a straight or curved drawn line

Sub-micron Accuracy

Sub-micron accuracy and unique computer controlled laser with guaranteed laser alignment

Safest Lasers on the Market

Lowest laser pulse energy for minimal energy near critical cells. Exclusion Zone™ feature ensures cell safety

Intuitive Software

Intuitive RI Viewer software package with streamlined interface. Programmable foot pedal controls software and laser functions including the new biopsy mode

Versatile

Compatible with all popular brands of micromanipulators and inverted microscopes

Saturn 5TM – Totally Committed To Embryo Safety

Multiple safety features reassure you that the Saturn™ laser is the safest laser on the market.

To keep your embryos safe, features include the Exclusion Zone which can be set according to distance or temperature.

By employing a higher laser power, the Saturn™ laser applies less total energy to make a specified hole size, in comparison to lower power laser systems.

Applications*

Using Saturn 5 Active™, you will find the following procedures are almost effortless and can be performed quickly and accurately.  These procedures may require difficult embryo manipulation when performed using fixed lasers.

Using a Saturn 5 Active™ has clear advantages to the welfare of the embryo.

Blastomere Biopsy, Polar Body Biopsy, Blastocyst Collapsing (for vitrification)

The Saturn 5 Active™ directional laser allows the embryo to stay in the desired position and focus so that ablations can be made wherever required without additional manipulation.  Our Biopsy Mode also allows safe multi-pulse drilling along a predetermined line.

Assisted Hatching

The directional laser means that accurate ablations can be made without the need to hold the embryo, making it very quick and accurate, with no additional consumable costs.

*The applicability of procedures is dependent on the regulations of the country into which the device is sold.

Blastocyst/Trophectoderm Biopsy

The Saturn 5 Active™ is unparalled in its ease of use.

The directional laser allows the user to make multiple ablations across the trophectoderm cells without needing to move the blastocyst.

This gives the user superb accuracy, safety and incredible speed.

Specifications

Pilot Laser
630-650nm spot targeting solid state diode laser – red pilot beam guarantees the position of the invisible ablation laser. It is impossible for the pilot and ablation lasers to be out of alignment with each other
Objective
Custom designed objective for optimum laser transmission, crystal clear imaging and minimal laser pulse times. Tested and proven not to exhibit astigmatism with Saturn 5™ Laser Systems. 20x and 40x objectives are available
Ablation Laser
1480nm / 400mW solid state diode laser. Pulse length range 0.005-2.0ms / 5-2000µs. Class 1 laser product. Tested and proven not to exhibit thermal lensing
Laser unit dimensions: (WxDxH)
220mm x 180mm x 34mm
CRi Oosight™ Compatibility
Compatible with CRI Oosight™ and SpindleView™ systems

Fluorescence Compatibility
Saturn™ Lasers Systems are compatible with epi-fluorescence on selected microscopes. Contact us for details
Operation Software
RI Viewer™ imaging software included – with digital laser targeting
PC System Requirements
Operating systems: Windows 8.1, Windows 8, Windows 7 (SP1), Windows Vista, Windows XP (SP3)
Mains Input
100-240VAC, 50-60Hz

Order Codes

Please use the table below to select the correct part number for your equipment.

For Saturn Fixed , other microscopes and any other system not covered by the part numbers below, contact RI Orders by emailing orders@research-instruments.com

Microscope Manufacturer Model Stage Objective Choice Part Number Description
Nikon Ti-S, Ti-U, Ti-E Integra Ti 40x 6-47-500-01 Saturn 5 Active Nikon Ti with Integra 40x
Nikon Ti-S, Ti-U, Ti-E Nikon XY 40x 6-47-500-02 Saturn 5 Active Nikon Ti with Nikon XY Stage 40x
Nikon Ti-S, Ti-U, Ti-E Integra Ti 20x 6-47-500-04 Saturn 5 Active Nikon Ti with Integra 20x
Nikon Ti-S, Ti-U, Ti-E Nikon XY 20x 6-47-500-05 Saturn 5 Active Nikon Ti with Nikon XY Stage 20x
Nikon TE2000 Integra Ti 40x 6-47-500-19 Saturn 5 Active Nikon TE2000 with Integra 40x
Nikon TE2000 Nikon XY 20x 6-47-500-20 Saturn 5 Active Nikon TE2000 with Nikon XY Stage 20x
Nikon TE2000 Integra Ti 40x 6-47-500-21 Saturn 5 Active Nikon TE2000 with Integra 40x
Nikon TE2000 Nikon XY 20x 6-47-500-22 Saturn 5 Active Nikon TE2000 with Nikon XY Stage 20x
Nikon TE200, TE300 40x 6-47-500-17 Saturn 5 Active Nikon TE300 40x
Nikon TE200, TE300 20x 6-47-500-18 Saturn 5 Active Nikon TE300 20x
Nikon Diaphot 200/300 40x 6-47-500-23 Saturn 5 Active Nikon Diaphot 200/300 40x
Olympus IX53, IX73, IX83 40x 6-47-500-03 Saturn 5 Active Olympus IX3 40x
Olympus IX53, IX73, IX83 20x 6-47-500-06 Saturn 5 Active Olympus IX3 20x
Olympus IX51, IX71, IX81 Left-hand Motor1 40x 6-47-500-13 Saturn 5 Active Olympus IX2 40x LH Motor
Olympus IX51, IX71, IX81 Left-hand Motor1 20x 6-47-500-14 Saturn 5 Active Olympus IX2 20x LH Motor
Olympus IX51, IX71, IX81 Right-hand Motor1 40x 6-47-500-15 Saturn 5 Active Olympus IX2 40x RH Motor
Olympus IX51, IX71, IX81 Right-hand Motor1 20x 6-47-500-16 Saturn 5 Active Olympus IX2 20x RH Motor
Olympus IX50, IX70 40x 6-47-500-24 Saturn 5 Active Olympus IX1 40x
Leica DMi8 40x 6-47-500-09 Saturn 5 Active Leica DMi8 40x
Leica DMi8 20x 6-47-500-08 Saturn 5 Active Leica DMi8 20x
Zeiss Axio Observer Left-hand Motor1 40x 6-47-500-09 Saturn 5 Active Zeiss Axio Observer 40x LH Motor
Zeiss Axio Observer Left-hand Motor1 20x 6-47-500-10 Saturn 5 Active Zeiss Axio Observer 20x LH Motor
Zeiss Axio Observer Right-hand Motor1 40x 6-47-500-11 Saturn 5 Active Zeiss Axio Observer 40x RH Motor
Zeiss Axio Observer Right-hand Motor1 20x 6-47-500-12 Saturn 5 Active Zeiss Axio Observer 20x RH Motor

1Choose left or right according to XY stage handle position. The motor should normally be opposite the stage handle.

Saturn 5 Fixed Selection

Microscope Manufacturer Model Stage Objective Choice Part Number Description
Nikon TE2000, Ti 40x 6-47-501-01 Saturn 5 Fixed Nikon TE2000 & Ti 40x
Nikon TE300 20x 6-47-501-02 Saturn 5 Fixed Nikon TE300 40x
Leica DMi8 40x 6-47-501-03 Saturn 5 Fixed Leica DMi8 40x

All systems are supplied with four mains cables: UK, EU (CEE 7/7), USA and China/Australia

FAQs

Q. Why do I need a laser?
A. For Assisted Hatching, Blastomere Biopsy, Polar Body Biopsy and other procedures, it is necessary to make a hole in the zona pellucida. Using a laser to do this is more precise, safer and quicker than other methods. You may save a great deal in disposables such as Acid Tyrodes and pipettes, and the laser’s use will reduce the amount of time lab staff spend preparing for and performing procedures. One other very important factor is that lasers standardise laboratory procedures by reducing operator variability, which can be a big problem when, for example, using Acid Tyrodes for Assisted Hatching.

Q. What are the advantages of the RI system?
A. The Saturn 5 Active™ laser can be directed to where the hole is required, eliminating the need to drag the embryo across the bottom of the dish, which can be difficult and time-consuming. The Saturn 5 Active™ has a visible pilot laser to ensure the laser is aligned to the objective (so that “flaring” of the laser beam does not occur) and to check the position of the laser when required. It has a high power and ultra short pulse time combination to make the most energy-efficient holes with minimal heat transfer. The Saturn 5™ laser systems use shorter pulse times than any competitor system. It also comes with a sophisticated software package – RI Viewer™ – to record images and data for both lab and patient use.

Q. Why do I need a pilot laser?
A. The pilot laser ensures that the laser is not going to flare or spread heat where it is not required. Microscopes also have a number of moving parts which could affect the position of the laser, eg the nosepiece, camera and port selector. The only way to be certain of the position of the laser is to check it before firing. Using a pilot laser that travels down the same fibre-optic as the main laser, guarantees the position and reduces the need for additional service visits. RI lasers are calibrated to within 1µm accuracy.

Q. What is the difference between the Saturn™ pilot laser and other red spot targeting systems?
A. Misalignment between the pilot laser and the infrared laser is not possible in a Saturn™ laser as the two travel down the same fibre-optic cable. Other systems have to be separately aligned. Shape and focus quality of the Saturn™ laser can be checked. This is not possible with other systems.

Q. Other lasers can adjust the power as well as the pulse length. Isn’t this an advantage?
A. No. Our research shows that the most efficient way to deliver the laser energy is to use pulse length to control the hole size. This results in the lowest amount of heat reaching the embryo, therefore the lowest possible risk of thermal damage. Besides which, other laser systems do not allow the user to vary the power in clinical use, only in a non-clinical evaluation mode. Ask to see the white paper from RI if you would like to see the data.

Q. Isn’t a higher power laser dangerous for the user?
A. Our lasers are Class 1 (the safest level), as defined by international laser safety standards, so no special safety precautions are required. As an extra precaution we also fit an infrared filter to prevent any infrared laser energy reaching the operator.

Q. How do I know that the laser beam is not damaging the embryo?
A. We have worked with embryologists to assess the damage done to embryos at different pulse lengths and at varying distances from critical cells. Based on the results of the study* we developed an “Exclusion Zone™” that surrounds the laser target and ensures superfluous heat will not be applied to critical cells. This is more clinically relevant than providing a temperature as this is entirely theoretical and leaves the embryologist with a problem in deciding what temperature is safe.
*K. Chatzimeletiou et al, “Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrodes on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics”, Reproductive BioMedicine Online, Vol 11. No 6. 2005 697–710

Q. I have been told that an off-axis laser cannot focus properly. Is this true?
A. This only applies to a simple lens. The Saturn 5 Active™ objective is a complex compound lens which is corrected for astigmatism, so the laser is focused correctly everywhere in the range of movement. This is easily demonstrated by looking at the pilot laser diffraction rings as the beam is moved. Any focus error would be obvious as the ring pattern would visibly change. Furthermore it has always been a routine requirement that all parts of the field of view are in sharp focus. The same is true for the laser.

Q. Is the alignment of the laser difficult?
A. No. Initial alignment is carried out once on installation. The laser position should be checked before each procedure, and this takes 2 seconds. A full calibration can be done from time to time to ensure optimal alignment and this takes 30 seconds. This is a very simple procedure and worthwhile to guarantee the embryo is not about to be damaged.

Q. Can I transfer it to another microscope?
A. Yes, if it is the same model of microscope. If it is a different model then most parts can be transferred, but a different mount will be required. Use the fibre cleaning kit 6-46-590 for installation.

Q. Can I upgrade my Saturn 3™ to a Saturn 5™?
A. Yes, the Saturn 3™ and Saturn 3 Active™ can be upgraded to Saturn 5™ or Saturn 5 Active™ with the purchase of parts.

Q. How long will Saturn 3 and Saturn 3 Active™ parts be manufactured for?
A. We will be supporting the Saturn 3™ lasers for at least 7 years from the launch of the Saturn 5™ lasers.

Q. How long is the warranty and expected life-span of the laser?
A. Our warranty is one year from despatch of goods. The system is designed to work reliably for many years.

Q. How does RI provide technical support in terms of service and repairs?
A. RI prides itself on its customer service. It is very important to us that our equipment is working at its best and that customers are completely satisfied. If a part needs replacing, RI can usually send replacements in 1-2 working days to most parts of the world, or repair equipment back in the UK. Our service team and those from major distributors can carry out routine servicing annually or as required.

Contact your distributor or our technical support team on service@research-instruments.com or by telephone Monday to Thursday 08.00-16.30 GMT and Friday 08.00-15.30 GMT on +44 (0) 1326 372753.

Q. Who else uses the RI system?
A. We have happy customers all over the world. Please contact us for further details.

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