As the pioneer of moveable lasers for Assisted Reproductive Technology, the Saturn 5 Active™ laser is spearheading a revolution in hatching and biopsy techniques in laboratories all over the world.
The Saturn 5™ Biopsy Mode is helping you to significantly improve biopsying methods; potentially reducing procedure time, lowering the incidence of blastocyst collapse and eliminating the need to mechanically tear off cells.
Using Biopsy Mode, you can draw a straight or curved line along the sample, select the number and size of holes, then simply “fire”. The laser will ablate exactly along the chosen path, meaning you no longer need to move the holding pipette at all. It is that easy.
As the Saturn 5™ pilot laser travels down the same fibre optic path as the ablation laser, it guarantees consistent positioning.
You can calibrate and verify hole size and firing position with sub-micron accuracy simply and quickly, leaving more time for your procedures.
Biopsy mode allows accurate laser drilling along a straight or curved drawn line
Sub-micron accuracy and unique computer controlled laser with guaranteed laser alignment
Lowest laser pulse energy for minimal energy near critical cells. Exclusion Zone™ feature ensures cell safety
Intuitive RI Viewer software package with streamlined interface. Programmable foot pedal controls software and laser functions including the new biopsy mode
Compatible with all popular brands of micromanipulators and inverted microscopes
Multiple safety features reassure you that the Saturn™ laser is the safest laser on the market.
To keep your embryos safe, features include the Exclusion Zone which can be set according to distance or temperature.
By employing a higher laser power, the Saturn™ laser applies less total energy to make a specified hole size, in comparison to lower power laser systems.
Using Saturn 5 Active™, you will find the following procedures are almost effortless and can be performed quickly and accurately. These procedures may require difficult embryo manipulation when performed using fixed lasers.
Using a Saturn 5 Active™ has clear advantages to the welfare of the embryo.
The Saturn 5 Active™ directional laser allows the embryo to stay in the desired position and focus so that ablations can be made wherever required without additional manipulation. Our Biopsy Mode also allows safe multi-pulse drilling along a predetermined line.
The directional laser means that accurate ablations can be made without the need to hold the embryo, making it very quick and accurate, with no additional consumable costs.
*The applicability of procedures is dependent on the regulations of the country into which the device is sold.
The Saturn 5 Active™ is unparalled in its ease of use.
The directional laser allows the user to make multiple ablations across the trophectoderm cells without needing to move the blastocyst.
This gives the user superb accuracy, safety and incredible speed.
Please use the table below to select the correct part number for your equipment.
For Saturn Fixed , other microscopes and any other system not covered by the part numbers below, contact RI Orders by emailing email@example.com
|Microscope Manufacturer||Model||Stage||Objective Choice||Part Number||Description|
|Nikon||Ti-S, Ti-U, Ti-E||Integra Ti||40x||6-47-500-01||Saturn 5 Active Nikon Ti with Integra 40x|
|Nikon||Ti-S, Ti-U, Ti-E||Nikon XY||40x||6-47-500-02||Saturn 5 Active Nikon Ti with Nikon XY Stage 40x|
|Nikon||Ti-S, Ti-U, Ti-E||Integra Ti||20x||6-47-500-04||Saturn 5 Active Nikon Ti with Integra 20x|
|Nikon||Ti-S, Ti-U, Ti-E||Nikon XY||20x||6-47-500-05||Saturn 5 Active Nikon Ti with Nikon XY Stage 20x|
|Nikon||TE2000||Integra Ti||40x||6-47-500-19||Saturn 5 Active Nikon TE2000 with Integra 40x|
|Nikon||TE2000||Nikon XY||20x||6-47-500-20||Saturn 5 Active Nikon TE2000 with Nikon XY Stage 20x|
|Nikon||TE2000||Integra Ti||40x||6-47-500-21||Saturn 5 Active Nikon TE2000 with Integra 40x|
|Nikon||TE2000||Nikon XY||20x||6-47-500-22||Saturn 5 Active Nikon TE2000 with Nikon XY Stage 20x|
|Nikon||TE200, TE300||–||40x||6-47-500-17||Saturn 5 Active Nikon TE300 40x|
|Nikon||TE200, TE300||–||20x||6-47-500-18||Saturn 5 Active Nikon TE300 20x|
|Nikon||Diaphot 200/300||–||40x||6-47-500-23||Saturn 5 Active Nikon Diaphot 200/300 40x|
|Olympus||IX53, IX73, IX83||–||40x||6-47-500-03||Saturn 5 Active Olympus IX3 40x|
|Olympus||IX53, IX73, IX83||–||20x||6-47-500-06||Saturn 5 Active Olympus IX3 20x||Olympus||IX51, IX71, IX81||Left-hand Motor1||40x||6-47-500-13||Saturn 5 Active Olympus IX2 40x LH Motor|
|Olympus||IX51, IX71, IX81||Left-hand Motor1||20x||6-47-500-14||Saturn 5 Active Olympus IX2 20x LH Motor||Olympus||IX51, IX71, IX81||Right-hand Motor1||40x||6-47-500-15||Saturn 5 Active Olympus IX2 40x RH Motor|
|Olympus||IX51, IX71, IX81||Right-hand Motor1||20x||6-47-500-16||Saturn 5 Active Olympus IX2 20x RH Motor|
|Olympus||IX50, IX70||–||40x||6-47-500-24||Saturn 5 Active Olympus IX1 40x|
|Leica||DMi8||–||40x||6-47-500-09||Saturn 5 Active Leica DMi8 40x|
|Leica||DMi8||–||20x||6-47-500-08||Saturn 5 Active Leica DMi8 20x||Zeiss||Axio Observer||Left-hand Motor1||40x||6-47-500-09||Saturn 5 Active Zeiss Axio Observer 40x LH Motor|
|Zeiss||Axio Observer||Left-hand Motor1||20x||6-47-500-10||Saturn 5 Active Zeiss Axio Observer 20x LH Motor||Zeiss||Axio Observer||Right-hand Motor1||40x||6-47-500-11||Saturn 5 Active Zeiss Axio Observer 40x RH Motor|
|Zeiss||Axio Observer||Right-hand Motor1||20x||6-47-500-12||Saturn 5 Active Zeiss Axio Observer 20x RH Motor|
1Choose left or right according to XY stage handle position. The motor should normally be opposite the stage handle.
|Microscope Manufacturer||Model||Stage||Objective Choice||Part Number||Description||Nikon||TE2000, Ti||–||40x||6-47-501-01||Saturn 5 Fixed Nikon TE2000 & Ti 40x|
|Nikon||TE300||–||20x||6-47-501-02||Saturn 5 Fixed Nikon TE300 40x||Leica||DMi8||–||40x||6-47-501-03||Saturn 5 Fixed Leica DMi8 40x|
All systems are supplied with four mains cables: UK, EU (CEE 7/7), USA and China/Australia
Q. Why do I need a laser?
A. For Assisted Hatching, Blastomere Biopsy, Polar Body Biopsy and other procedures, it is necessary to make a hole in the zona pellucida. Using a laser to do this is more precise, safer and quicker than other methods. You may save a great deal in disposables such as Acid Tyrodes and pipettes, and the laser’s use will reduce the amount of time lab staff spend preparing for and performing procedures. One other very important factor is that lasers standardise laboratory procedures by reducing operator variability, which can be a big problem when, for example, using Acid Tyrodes for Assisted Hatching.
Q. What are the advantages of the RI system?
A. The Saturn 5 Active™ laser can be directed to where the hole is required, eliminating the need to drag the embryo across the bottom of the dish, which can be difficult and time-consuming. The Saturn 5 Active™ has a visible pilot laser to ensure the laser is aligned to the objective (so that “flaring” of the laser beam does not occur) and to check the position of the laser when required. It has a high power and ultra short pulse time combination to make the most energy-efficient holes with minimal heat transfer. The Saturn 5™ laser systems use shorter pulse times than any competitor system. It also comes with a sophisticated software package – RI Viewer™ – to record images and data for both lab and patient use.
Q. Why do I need a pilot laser?
A. The pilot laser ensures that the laser is not going to flare or spread heat where it is not required. Microscopes also have a number of moving parts which could affect the position of the laser, eg the nosepiece, camera and port selector. The only way to be certain of the position of the laser is to check it before firing. Using a pilot laser that travels down the same fibre-optic as the main laser, guarantees the position and reduces the need for additional service visits. RI lasers are calibrated to within 1µm accuracy.
Q. What is the difference between the Saturn™ pilot laser and other red spot targeting systems?
A. Misalignment between the pilot laser and the infrared laser is not possible in a Saturn™ laser as the two travel down the same fibre-optic cable. Other systems have to be separately aligned. Shape and focus quality of the Saturn™ laser can be checked. This is not possible with other systems.
Q. Other lasers can adjust the power as well as the pulse length. Isn’t this an advantage?
A. No. Our research shows that the most efficient way to deliver the laser energy is to use pulse length to control the hole size. This results in the lowest amount of heat reaching the embryo, therefore the lowest possible risk of thermal damage. Besides which, other laser systems do not allow the user to vary the power in clinical use, only in a non-clinical evaluation mode. Ask to see the white paper from RI if you would like to see the data.
Q. Isn’t a higher power laser dangerous for the user?
A. Our lasers are Class 1 (the safest level), as defined by international laser safety standards, so no special safety precautions are required. As an extra precaution we also fit an infrared filter to prevent any infrared laser energy reaching the operator.
Q. How do I know that the laser beam is not damaging the embryo?
A. We have worked with embryologists to assess the damage done to embryos at different pulse lengths and at varying distances from critical cells. Based on the results of the study* we developed an “Exclusion Zone™” that surrounds the laser target and ensures superfluous heat will not be applied to critical cells. This is more clinically relevant than providing a temperature as this is entirely theoretical and leaves the embryologist with a problem in deciding what temperature is safe.
*K. Chatzimeletiou et al, “Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrodes on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics”, Reproductive BioMedicine Online, Vol 11. No 6. 2005 697–710
Q. I have been told that an off-axis laser cannot focus properly. Is this true?
A. This only applies to a simple lens. The Saturn 5 Active™ objective is a complex compound lens which is corrected for astigmatism, so the laser is focused correctly everywhere in the range of movement. This is easily demonstrated by looking at the pilot laser diffraction rings as the beam is moved. Any focus error would be obvious as the ring pattern would visibly change. Furthermore it has always been a routine requirement that all parts of the field of view are in sharp focus. The same is true for the laser.
Q. Is the alignment of the laser difficult?
A. No. Initial alignment is carried out once on installation. The laser position should be checked before each procedure, and this takes 2 seconds. A full calibration can be done from time to time to ensure optimal alignment and this takes 30 seconds. This is a very simple procedure and worthwhile to guarantee the embryo is not about to be damaged.
Q. Can I transfer it to another microscope?
A. Yes, if it is the same model of microscope. If it is a different model then most parts can be transferred, but a different mount will be required. Use the fibre cleaning kit 6-46-590 for installation.
Q. Can I upgrade my Saturn 3™ to a Saturn 5™?
A. Yes, the Saturn 3™ and Saturn 3 Active™ can be upgraded to Saturn 5™ or Saturn 5 Active™ with the purchase of parts.
Q. How long will Saturn 3 and Saturn 3 Active™ parts be manufactured for?
A. We will be supporting the Saturn 3™ lasers for at least 7 years from the launch of the Saturn 5™ lasers.
Q. How long is the warranty and expected life-span of the laser?
A. Our warranty is one year from despatch of goods. The system is designed to work reliably for many years.
Q. How does RI provide technical support in terms of service and repairs?
A. RI prides itself on its customer service. It is very important to us that our equipment is working at its best and that customers are completely satisfied. If a part needs replacing, RI can usually send replacements in 1-2 working days to most parts of the world, or repair equipment back in the UK. Our service team and those from major distributors can carry out routine servicing annually or as required.
Contact your distributor or our technical support team on firstname.lastname@example.org or by telephone Monday to Thursday 08.00-16.30 GMT and Friday 08.00-15.30 GMT on +44 (0) 1326 372753.
Q. Who else uses the RI system?
A. We have happy customers all over the world. Please contact us for further details.